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Human mesenchymal stem/stromal cells (h MSCs) are of great interest in the field of regenerative medicine.
Their therapeutic properties can be mainly attributed to their secretome, which has been shown to modulate several processes in vitro and in vivo, such as cell proliferation, survival, differentiation, immunomodulation, anti-apoptosis, angiogenesis and stimulation of tissue adjacent cells.
PPRF-msc6) was able to support the rapid and efficient isolation and expansion of h MSCs from different sources.
In addition to developing a well-defined medium, we have also developed a scalable, computer-controlled stirred suspension bioreactor-based microcarrier-mediated bioprocess that can be translated to operate in a closed system.
Herein, we present data indicating that the use of computer-controlled suspension bioreactors enhanced the neuroregulatory profile of h MSCs secretome.Using stirred suspension bioreactors, a number of advantages can be achieved including: (1) a large number of cells can be expanded in one vessel (minimizing vessel-to-vessel variability and minimizing cost related to labor and consumables), (2) the bioreactors can be operated in a fed-batch or perfusion mode of operation (removing metabolites and inhibitory factors while replenishing growth factors) and (3) the bioreactors can be set up with computer-controlled online monitoring instruments to ensure tight control of process variables such as p H, temperature and dissolved oxygen concentration.Additionally, it has been shown that h MSCs respond to changes in their physiological environment.In recent years it has been shown that the therapeutic benefits of human mesenchymal stem/stromal cells (h MSCs) in the Central Nervous System (CNS) are mainly attributed to their secretome.
The implementation of computer-controlled suspension bioreactors has shown to be a viable route for the expansion of these cells to large numbers.
In line with these findings, the same trend was also observed in the Bioplex based analysis to the h BM-MSCs dynamic secretome concerning the expression of classical trophic factors such as BDNF (t = 1.926, p = 0.126), VEGF (t = 1.995, p = 0.110), NGF (t = 3.055, 0.091), and IGF-1 (t = 31,78, p CM analysis shows that the pattern of protein expression is modulated when we change from (A) static to (B) dynamic culture condition.